3′,4′-Dihydroxyflavonol improves post-ischaemic coronary endothelial function following 7 days reperfusion in sheep

Abstract

3′,4′-dihydroxyflavonol (DiOHF) is a potent antioxidant that reduces infarct size following myocardial ischaemia-reperfusion. Since oxidative stress induced by myocardial ischaemia-reperfusion impairs endothelium-dependent vasodilatation, we investigated whether DiOHF preserved coronary endothelial function following ischemia-reperfusion. One week after surgery conscious, instrumented sheep were subjected to 1 h of myocardial ischaemia followed by 7 days reperfusion. Immediately before reperfusion, sheep were injected with DiOHF (2 mg/kg iv, n = 4) or vehicle (dimethyl sulphoxide, n = 4). Coronary vascular responses to the endothelium-dependent vasodilator acetylcholine (ACh, 0.05-10.0 μg/kg..